v 730 jasco spectrophotometer  (JASCO Inc)

Journal: Advanced Science

Article Title: Blood Cell‐Camouflaged Liquid Metal Nanoconjugates Orchestrate Treg Depletion and STING‐Amplified Photothermal Immunity for Metastatic Triple‐Negative Breast Cancer Therapy

doi: 10.1002/advs.77069

Figure Lengend Snippet: Comprehensive characterization of blood‐camouflaged liquid metal nanocomposites. (A) Representative digital photograph of B–LM–DMX–αCD25 nanocomplex suspension showing homogeneous dark‐red coloration indicative of stable colloidal dispersion. The uniform appearance contrasts sharply with the opaque gray, aggregated suspensions observed for pristine LM and DSPE–LM (Figure ), demonstrating superior dispersibility conferred by whole‐blood biomimetic engineering. (B) High‐resolution transmission electron microscopy (TEM) image of B–LM–DMX–αCD25 (after DMX encapsulation and anti‐CD25 conjugation) nanocomposites revealing well‐defined core–shell architecture. The electron‐dense liquid metal core (∼80 nm diameter) is encapsulated by a continuous biomimetic membrane coating (∼15 nm thickness), confirming successful blood component‐mediated surface camouflaging. (C) Dynamic light scattering (DLS) size distribution profiles of B–LM–DMX and B–LM–DMX–αCD25 measured on day 7 post‐synthesis, demonstrating excellent colloidal stability over time. The hydrodynamic diameters are ∼155 nm (B–LM–DMX) and ∼171 nm (B–LM–DMX–αCD25) with narrow polydispersity (PDI < 0.2). The modest size increase (∼17 nm) following anti‐CD25 conjugation provides direct evidence of successful antibody functionalization. (D) Zeta potential measurements showing progressive surface charge modulation during sequential functionalization: pristine LM (−14 mV), B–LM–DMX (−28 mV), and B–LM–DMX–αCD25 (−18 mV). The shift toward less negative values after antibody conjugation confirms successful surface modification while maintaining colloidal stability. Data represent mean ± SEM ( n = 3 independent measurements). (E) UV–vis–NIR absorption spectra of B–LM–DMX–αCD25, B–LM, B–LM–αCD25, DSPE–LM, DMXAA, and whole blood components (left panel), and expanded NIR region spectra (700–900 nm) of B–LM–DMX–αCD25 and DSPE–LM (right panel). The full spectra confirm successful co‐assembly of all functional components, with characteristic absorption features of blood‐derived proteins (∼280 nm), hemoglobin derivatives (∼410 nm), and DMX (∼340 nm). The expanded NIR spectra reveal that B–LM–DMX–αCD25 exhibits broad, homogeneous absorption across the 700–900 nm window, reflecting its excellent colloidal stability and uniform particle distribution. In contrast, the apparently elevated NIR absorbance of DSPE–LM is attributable to light scattering artifacts arising from poor colloidal stability and macroscopic particle aggregation (Figure ), rather than intrinsic NIR absorption capacity, and therefore does not accurately represent its photothermal conversion potential. The superior colloidal homogeneity of B–LM–DMX–αCD25 ensures that its measured NIR absorbance accurately reflects true light absorption, providing a reliable basis for photothermal performance prediction. (F) Near‐infrared laser‐responsive DMX release kinetics from B–LM–DMX–αCD25 nanocomposites. Minimal spontaneous leakage (<5%) occurs without laser stimulation, confirming excellent cargo retention during circulation. Upon 808 nm laser irradiation (1.0 W, ∼51 mW mm − 2 ), rapid burst release achieves nearly 90% cumulative DMX liberation within 15 min, demonstrating on‐demand, spatiotemporally controlled drug discharge synchronized with photothermal ablation. Data are presented as mean ± SEM ( n = 3 independent experiments). Cumulative DMX release values were calculated by subtracting the baseline spontaneous leakage measured under physiological conditions (PBS, pH 7.4, 37°C) without laser irradiation. Spontaneous leakage remained below 5% throughout the observation period, confirming excellent cargo retention during systemic circulation prior to laser activation.

Article Snippet: Optical absorption spectra were recorded using a UV–vis–NIR spectrophotometer (Model V‐730 BIO; Jasco Corporation, Tokyo, Japan) over the wavelength range of 300–900 nm.

Techniques: Suspension, Dispersion, Transmission Assay, Electron Microscopy, Encapsulation, Conjugation Assay, Membrane, Zeta Potential Analyzer, Modification, Functional Assay, Derivative Assay, Irradiation, Activation Assay

Journal: Advanced Science

Article Title: Blood Cell‐Camouflaged Liquid Metal Nanoconjugates Orchestrate Treg Depletion and STING‐Amplified Photothermal Immunity for Metastatic Triple‐Negative Breast Cancer Therapy

doi: 10.1002/advs.77069

Figure Lengend Snippet: Superior photothermal conversion performance and stability of B–LM nanocomposites. (A) Temperature elevation profiles of B–LM suspensions at various liquid metal concentrations (100–500 µg mL − 1 ) under 808 nm near‐infrared laser irradiation at different power densities: 0.5 W (∼25.5 mW mm − 2 ), 1.0 W (∼51 mW mm − 2 ), and 1.5 W (∼76.5 mW mm − 2 ). Temperature increases in a concentration‐ and power‐dependent manner, reaching >60°C within 5 min at 500 µg mL − 1 LM under 1.5 W irradiation—well exceeding the thermal threshold for irreversible protein denaturation and tumor cell apoptosis. Milli‐Q water (control) shows negligible temperature change (<5°C), confirming that hyperthermia originates exclusively from liquid metal photothermal conversion. Data represent mean ± SD ( n = 3). (B) Representative infrared thermographic images of B–LM suspensions at three concentrations (125, 250, and 500 µg mL − 1 ) under three 808 nm laser power settings (0.5, 1.0, and 1.5 W), captured after 5 min of continuous irradiation. False‐color images show spatial heat distribution (blue = low; red = high temperature). Both increasing concentration and laser power progressively elevate local temperature, demonstrating concentration‐ and power‐dependent photothermal tunability. Heat generation remains highly localized to the irradiated area, confirming spatial precision essential for minimizing collateral thermal damage in vivo. (C) UV–vis–NIR absorption spectra of B–LM nanocomposites recorded before and after 808 nm laser irradiation (1.5 W, ∼76.5 mW mm − 2 , 5 min), showing no detectable spectral shifts or intensity attenuation. This exceptional photostability—contrasting sharply with organic dye‐based photothermal agents that typically exhibit rapid photobleaching—confirms structural integrity and resistance to photodegradation, supporting reliability for repeated therapeutic applications. (D) Cyclic heating–cooling stability assessment across five consecutive on/off cycles (5 min laser irradiation at 1.5 W followed by 5 min natural cooling per cycle). Temperature profiles remain highly reproducible with no observable decline in peak temperature or heating rate, confirming excellent thermal endurance and suitability for fractionated treatment protocols. LM concentration: 500 µg mL − 1 in 0.5 mL PBS.

Article Snippet: Optical absorption spectra were recorded using a UV–vis–NIR spectrophotometer (Model V‐730 BIO; Jasco Corporation, Tokyo, Japan) over the wavelength range of 300–900 nm.

Techniques: Irradiation, Concentration Assay, Control, In Vivo